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KMID : 0880220120500050860
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Journal of Microbiology
2012 Volume.50 No. 5 p.860 ~ p.863
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Next-generation sequencing-based genome-wide mutation analysis of l-lysine-producing Corynebacterium glutamicum ATCC 21300 strain
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Lee Chang-Soo
Nam Jae-Young
Son Eun-Suk
Kwon O-chul
Han Woo-Ri-Ja-Rang
Park Young-Jin
Cho Jae-Yong
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Abstract
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In order to identify single nucleotide polymorphism and insertion/deletion mutations, we performed whole-genome re-sequencing of the enhanced l-lysine-producing Corynebacteriumglutamicum ATCC 21300 strain. In total, 142 single nucleotide polymorphisms and 477 insertion/deletion mutations were identified in the ATCC 21300 strain when compared to 3,434 predicted genes of the wild-type C. glutamicum ATCC 13032 strain. Among them, 110 transitions and 29 transversions of single nucleotide polymorphisms were found from genes of the ATCC 21300 strain. In addition, 11 genes, involved in the L-lysine biosynthetic pathway and central carbohydrate metabolism, contained mutations including single nucleotide polymorphisms and insertions/deletions. Interestingly, RT-PCR analysis of these 11 genes indicated that they were normally expressed in the ATCC 21300 strain. This information of genome-wide gene-associated variations will be useful for genome breeding of C. glutamicum in order to develop an industrial amino acid-producing strain with minimal mutation.
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KEYWORD
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mutation, SNP, InDel, genome, Corynebacteriumglutamicum
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